Tables

Table 1
Black River Sample Stations and Sample Types*

River Mile	Location	Sampling Type
0.3	Harbor mouth at boat gas station	S, LT
0.5	Ust. Erie St. Dst. Kobe	LT, FT
	West bank
2.4	Kobe (dst dredging)	S, LT, FT
	East bank, lake side of Turning Basin
2.5	East bank, Turning Basin	LT
2.9	Ust Turning Basin, West Bank of Kobe	S, LT, D
3.6-4.8	Kobe area. Broad area sampling for snails	D
4.6	West Bank of Kobe near coal pile	S
4.9	Mouth of French Creek	LT, FT
2.5 (Tributary)	Kline Ditch:tributary of French Creek
	@ RM 3.9	D
5.2	Ust. Kobe and French Creek	S, LT, Ft, D
8.3	St. Rt. 254	D
9.8	Ford Rd. (Dst. Elyria WWTP)	S, LT
11.6	Spring Valley Country Club
	(Ust. Elyria WWTP)	S, LT,FT, D
15.0	Dst. confluence East and West Branches at Cascade Park	LT, FT, D
18.9 (east Br)	Ust. Grafton (East Fk, East Branch)	LT, FT
Not Applicable	Field Laboratory Controls	Ft
	Laboratory Controls	LT

Sampling Codes:	S-sediment chemistry by Ohio EPA and USGS LT-laboratory sediment toxicity FT-field (in situ) toxicity (water and sediment) D-DNA fingerprinting of snails

Table 2
In Situ Exposures on the Black River, Fall 1997

Black River--In situ Survivals
September 29-October 2, 1997
	Hotel cont.	0.3 Mi (U)	0.3 mi (L)	0.5 Mi (U)	0.5 Mi (L)

H. azteca	10	0	9	7	10
	10	9	0	10	7
	10	0	7	6	8
	10	9	5	8	8

Ave. surviv	10	4.5	5.25	7.75	8.25
% surviv	100	45	52.5	77.5	82.5
St. dev.	0	5.1962	3.8622	1.7078	1.2583
Coeff. var	0	115.470054	73.5659062	22.0364533	15.2521908

C. tentans	9	6	6	6	8
	10	8	10	6	6
	9	10	8	8	10
	9	8	11	9	8

Ave. surviv	9.25	8	8.75	7.25	8
% surviv	92.5	80	87.5	72.5	80
St. dev.	0.5	1.6330	2.2174	1.5	1.6330
Coeff. var	5.4054	20.4124	25.3412	20.6897	20.4124

P. promelas	10	9	6	0	10
	10	10	10	0	10
	10	10	10	9	9
	10	3	9	7	10

Ave. surviv	10	8.000	8.750	4	9.75
% surviv	100	80.000	87.500	40	97.5
St. dev.	3.8622	1.8930	1.8930	0.5	3.3665
Coeff. var	38.6221	23.6621	21.6339	12.5000	34.5282

C. dubia	9		11		5
	10		10		7
	10
	9

Ave. surviv	9.25		10.5		6
% surviv	92.5		105		60
St. dev.	0.9574		0.7071		1.4142
Coeff. var	10.3506		6.7344		23.5702

	2.4 (U)	2.4 (L)	5.2 (U)

H.azteca	5	7	5
		10	9
		10	9
		10	11

Ave. surviv	5	9.25	8.5
% surviv	50	92.5	85
St. dev.	NA	1.5	2.5166
Coeff. var	NA	16.2162162	29.6071939

C. tentans	9	10	10
		9	10
		9	10
		8	7

Ave. surviv	9	9	9.25
% surviv	90	90	92.5
St. dev.	0	0.8165	1.5
Coeff. var	0.0000	9.0722	16.2162

P. promelas	0	9	7
	7	10	9
		10	9
		10	8

Ave. surviv	3.5	9.75	8.25
% surviv	35	97.5	82.5
St. dev.	0.5	4.6904	1.8930
Coeff. var	14.2857	48.1068	22.9451

C. dubia		8
		7

Ave. surviv		7.5
% surviv		75
St. dev.		0.7071
Coeff. var		9.4281

	5.2 (L)	15.0 Mi	18.9 Mi

H.azteca	7	10	7
	5	9	9
	10	10	10
	9	7	11

Ave. surviv	7.75	9	9.25
% surviv	77.5	9	9.25
St. dev.	2.2174	1.4142	1.7078
Coeff. var	28.6110424	15.713484	18.4629744

C. tentans	8	9	7
	10	8	8
	9	10	10
	11	8	10

Ave. surviv	9.5	8.75	8.75
% surviv	95	87.5	87.5
St. dev.	1.2910	0.9574	1.5
Coeff. var	13.5894	10.9420	17.1429

P.promelas	6	9	10
	9	10	10
	10	2	8
	10	10	5

Ave. surviv	8.75	7.75	8.25
% surviv	87.5	77.5	82.5
St. dev.	1.8930	2.3329	0.9574
Coeff. var	21.6339	30.4891	11.6052

C. dubia	9	11	10
	9

Ave. surviv	9	11	10
% surviv	90	110	100
St. dev.	0.0000	0.0000	0.0000
Coeff. var	0.0000	0.0000	0.0000

U=upper level-water L=Lower level-sediment ***Chamber mesh broken

Table 3
Laboratory Sediment Toxicity
on the Black River, Fall 1997

Black River 10 Day Laboratory Test-Survivals
October 31, 1997-November 10, 1997
			0-2 cm	8-10 cm	0-2 cm
	Florissant	18.9 Mi (EB)	0.3 Mi (U)	0.3 Mi (L)	0.5 Mi (U)

H.azteca	8	7	1	6	7
	7	7	3	5	5
	5	8	3	5	5
	7	7	6	6	6

Ave.surviv	6.75	7.25	3.25	5.5	5.75
% surviv	84.375	90.625	40.625	68.75	71.875
St. dev.	1.26	0.50	2.06	0.58	0.96
Coeff. var	18.64	6.90	63.43	10.50	16.65

C. tentans	8	10	7	3
	10	7	6	3
	9	10	7	6
	8	8	6	7

Ave.surviv	8.75	8.75	6.5	4.75	0
% surviv	87.5	87.5	65	47.5	0
St. dev.	0.96	1.50	0.58	2.06
Coeff. var	10.94	17.14	8.88	43.40

	8-10 cm	0-2 cm	8-10 cm	0-2 cm	8-10 cm
	0.5 Mi (L)	2.4 Mile (U)	2.4 Mile (L)	2.5 Mile (U	2.5 Mile (L)

H.azteca	8	10	6	6	2
	7	8	3	2	2
	7	7	4	2	0
	6	6	6	2	2

Ave.surviv	7	7.75	4.75	3	1.5
% surviv	87.5	96.875	59.375	37.5	18.75
St. dev.	0.82	1.71	1.50	2.00	1.00
Coeff. var	11.66	22.04	31.58	66.67	66.67

C. tentans		8	6	10	6
		6	8	8	7
		4	5	6	10
		10	6	8	7

Ave.surviv	0	7	6.25	8	7.5
% surviv	0	70	62.5	80	75
St. dev.		2.58	1.26	1.63	1.73
Coeff. var		39.89	20.13	20.41	23.09

	0-2 cm	8-10 cm	0-2 cm	8-10 cm	0-2 cm
	2.9 Mile (U)	2.9 Mile (L)	4.6 Mile (U)	4.6 Mile (L)	5.2 Mile (U)

H.azteca	5	6	8	6	7
	8	3	8	6	8
	4	5	5	7	8
	7	9	7	6	8

Ave.surviv	6	5.75	7	6.25	7.75
% surviv	75	71.875	87.5	78.125	96.875
St. dev.	1.83	2.50	1.41	0.50	0.50
Coeff. var	30.43	43.48	20.20	8.00	6.45

C. tentans			7	4	7
			6	7	8
			4	5	7
			7	5	8

Ave.surviv	0	0	6	5.25	7.5
% surviv	0	0	60	52.5	75
St. dev.			1.41	1.26	0.58
Coeff. var			23.57	23.97	7.70

	8-10 cm
	5.2 Mile (L)	9.8 Mi	11.6 Mi	15.0 Mi

H.azteca	5	6	6	8
	6	7	6	8
	7	7	5	2
	8	11	6	8

Ave.surviv	6.5	7.75	5.75	6.5
% surviv	81.25	96.875	71.875	81.25
St. dev.	1.29	2.22	0.50	3.00
Coeff. var	19.86	28.61	8.70	46.15

C. tentans	10	3	8	1
	8	4	8	6
	7	6	9	3
	6	6	7	2

Ave.surviv	7.75	4.75	8	3
% surviv	77.5	47.5	80	30
St. dev.	1.71	1.50	0.82	2.16
Coeff. var	22.04	31.58	10.21	72.01

Black River 10 Day Laboratory Test-Dry Weights
October 31, 1997-November 10, 1997
			0-2 cm	8-10 cm	0-2 cm
	Florissant	18.9 Mi (EB)	0.3 Mi (U)	0.3 Mi (L)	0.5 Mi (U)

H.azteca	0.0628571	0.18714	0.138	0.08	0.06
	0.0542857	0.30143	0.076	0.14667	0.145
	0.044	0.252	0.102	0.08	0.12
	0.1222	0.19	0.10667	0.07333	0.12667

Ave.dry wt	0.708357	0.236425	0.1056675	0.95	0.1129175
St. dev.	0.04	0.05	0.03	0.03	0.04
Coeff. var	49.55	23.54	24.07	36.41	32.61

C. tentans	1.7025	1.95	1.32986	0.46333
	1.782	2.33143	1.364	0.658
	1.3025	1.535	0.94714	0.838333
	1.6725	2.03	1.91833	0.515

Ave. dry wt	1.614875	1.9616075	1.4055825	0.6186658	0
St. dev.	0.21	0.33	0.40	0.17
Coeff. var	13.21	16.74	28.31	27.16

	8-10 cm	0-2 cm	8-10 cm	0-2 cm	8-10 cm
	0.5 Mi (L)	2.4 Mile (U)	2.4 Mile (L)	2.5 Mile (U	2.5 Mile (L)

H.azteca	0.115	0.1075	0.05	0.06167	0.095
	0.23333	0.1025	0.03	0.06	0.125
	0.07714	0.10714	0.0975	0.195	0.22
	0.08667		0.85

Ave. dry wt	0.128035	0.10571333	0.065625	0.10555667	0.1466667
St. dev.	0.07	0.00	0.03	0.08	0.07
Coeff var	56.25	2.63	47.42	73.38	44.49

C. tentans		0.942	1.4667	1.181	1.11429
		1.44375	1.83	0.83125	1.24714
		1.00143	3.33333	0.92167	0.915
		0.93667	1.00333	0.575	1.544

Ave. dry wt	0	1.0809625	1.9083325	0.87723	1.2051075
St. dev.		0.24	1.01	0.25	0.26
Coeff. var		22.54	52.84	28.51	21.90

	0-2 cm	8-10 cm	0-2 cm	8-10 cm	0-2 cm
	2.9 Mile (U)	2.9 Mile (L)	4.6 Mile (U)	4.6 Mile (L)	5.2 Mile (U)

H.azteca	0.112	0.09667	0.1475	0.8833	0.09286
	0.04125	0.188	0.08375	0.12833	0.10375
	0.1175	0.01	0.098	0.10143	0.06429
	0.09054	0.09889	0.11	0.10167	0.12125

Ave. dry wt	0.0903225	0.9839	0.1098125	0.10494	0.0955375
St. dev.	0.03	0.07	0.03	0.02	0.02
Coeff. var	38.44	73.87	24.88	16.00	25.01

C. tentans			1.85429	1.58571	1.5574
			2.08333	1.822	1.72
			1.284	2.3675	1.76514
			3.5075		1.55375

Ave. dry wt	0	0	2.18228	1.92507	1.6486575
St. dev.			0.95	0.40	0.11
Coeff. var			43.31	20.83	6.67

	8-10 cm
	5.2 Mile (L)	9.8 Mi	11.6 Mi	15.0 Mi

H.azteca	0.09	0.114	0.0725	0.15375
	0.02667	0.0917	0.215	0.08125
	0.08	0.09	0.0725	0.09
	0.11375	0.1756	0.195	0.11875

Ave. dry wt	0.077605	0.1178	0.1388	0.1109
St. dev.	0.04	0.04	0.08	0.03
Coeff. var	47.40	33.98	55.45	29.50

C. tentans	1.81	0.8167	1.28625	0.82
	2.335	0.915	1.425	1.795
	1.86	0.7533	1.18111	1.08333
	2.375	0.575	1.47571	2.885

Ave. dry wt	2.095	0.765	1.342	1.6458
St. dev.	0.30	0.14	0.13	0.92
Coeff. var	14.38	18.70	9.98	56.09

Table 4 *RAPD Genetic Similarity in the Snail, Physella gyrina,* on the Black River, Fall 1997**
Station (River Mile)	Mean Genetic Similarity (Std. Dev.)
2.9	0.821 (0.043)
3.6-4.8	0.810 (0.045)
5.2	0.853 (0.048)
Tributary (2.5) of French Creek (4.9)	0.882 (0.041)
15.0	0.815 (0.061)
18.9 (East Br.)	0.831 (0.042)

Table 5
In Situ Exposures in the Black River, Spring 1998

*Black River--In situ* Survivals**
11 June - 13 June, 1998

	Hotel cont	0.5 Mi	2.4 Mi	4.9 Mi


H. azteca	10	10	9	9
	8	10	8	10
	7	9	9	9
	7	10	10	10

Ave. surviv	8	9.75	9	9.5
% surviv	80	97.5	90	95
St. dev.	1.4142136	0.5000	0.8165	0.5774
Coeff. var	17.67767	5.1282051	9.0721842	6.0773713


C. tentans	10	8	6	10
	4	9	8	8
	9	9	10	7
	8	9	9	8

Ave. surv	7.75	8.75	8.25	8.25
% surviv	77.5	87.5	82.5	82.5
St. dev.	2.6299556	0.5	2	1.3
Coeff. var	33.9349	5.7143	20.7009	15.2522


P. promelas	10	8		9
	10	10	9	10
	10	9	9	6
	10	7	7	6

Ave. surviv	10	8.5	8.3333333	7.75
% surviv	100	85	83.333333	77.5
St. dev.	0	1.2909944	1.1547005	2.0615528
Coeff. var	0	15.18817	13.856406	26.600681


C. dubia	15	8	9	8
	2	9	8	9
	1	10	8	9
	7	8	8	8

Ave. surviv	6.25	8.75	8.25	8.5
% surviv	62.5	87.5	82.5	85
St. dev.	6.3966	0.9574	0.5000	0.5774
Coeff. var	102.3458	10.9420	6.0606	6.7924


	5.2 Mi	15.0 Mi	18.9 Mi


H. azteca	10	10	10
	10	10	10
	10	9	9
	8	10	10

Ave. surviv	9.5	9.75	9.75
% surviv	95	97.5	97.5
St. dev.	1.0000	0.5000	0.5000
Coeff. var	10.526316	5.1282051	5.1282051


C. tentans	7	9	8
	8	10	6
	9	8	5
	8	10	9

Ave. surv	8	9.25	7
% surviv	80	92.5	70
St. dev.	0.8	0.9574	1.8257
Coeff. var	10.2062	10.3506	26.0820


P. promelas	9	10	10
	7	10	10
	9	10	9
		10	8

Ave. surviv	8.3333333	10.000	9.250
% surviv	83.333333	100.000	92.500
St. dev.	1.1547005	0	0.9574271
Coeff. var	13.856406	0	10.350563


C. dubia	10	9	13
	10	10	4
	9	8	9
	10	10	17

Ave. surviv	9.75	9.25	10.75
% surviv	97.5	92.5	107.5
St. dev.	0.5000	0.9574	5.5603
Coeff. var	5.1282	10.3506	51.7235

Table 6
Concentrations of organic compounds in sediments from the
Black River, 1997 All concentrations in ug/kg

Compound (CAS#)	River Mile
	11.60	9.81	5.20	4.80	2.90	2.90 dup	2.32	0.42
Semi-volatile Organics
Benzo(a)anthracene (56-55-3(	<0.5	<0.5	<0.7	<0.9	0.8	<0.7	1.6	<0.7
Benzo(a)pyrene (50-32-8)	<0.5	<0.5	<0.7	<0.9	0.8	<0.7	1.7	<0.7
Benzo(b)fluoranthene (205-99-2)	<0.5	<0.5	<0.7	0.9	0.9	0.7	12.0	<0.7
Benzo(g,h,i)perylene (191-24-2)	<0.5	<0.5	<0.7	<0.9	<0.8	<0.7	1.7	<0.7
benzo(k)fluoranthene (207-08-9)	<0.5	<0.5	<0.7	<0.9	<0.8	<0.7	1.5	<0.7
bis (2-ethylhexyl) phthalate (117-81-7)	<0.5	<0.5	0.7	1.2	<0.8	<0.7	<0.7	1.5
Chrysene (218-01-9)	0.6	<0.5	0.7	1.1	1.1	1.0	2.0	<0.7
Fluoranthene (206-44-0)	1.1	0.8	1.2	1.7	2.0	1.6	2.2	<0.7
Indeno(1,2,3-cd)pyrene (193-39-5)	<0.5	<0.5	<0.7	<0.9	0.8	<0.7	1.8	<0.7
3 & 4 Methylphenol (106-44-5)	<0.5	<0.5	<0.7	1.9	1.1	1.3	<0.7	<0.7
Naphthalene (91-20-3)	<0.5	<0.5	<0.7	<0.9	<0.8	<0.7	0.7	<0.7
Phenanthrene (85-01-8)	0.7	0.6	<0.7	<0.9	1.2	0.9	1.0	<0.7
Pyrene (129-00-0)	1.0	0.7	1.0	1.4	1.6	1.4	1.7	<0.7
Volatile Organics
Acetone (67-64-1)	<0.7	<0.6	<0.8	0.1	<0.13	0.1	<0.9	<0.9
Toulene (108-88-3)	<0.6	<0.5	<0.6	<0.9	1.0	1.0	<0.7	<0.7
Organochlorine Pesticides and PCB's
4,4'-DDE (72-55-9)	<5.7	<4.9	<7.1	10	<7.6	<7.8	<7	<7.4
4,4'-DDT (50-29-3)	<5.7	<4.9	<7.1	9.8	<7.6	<7.8	<7	<7.4
PCB-1254 (11097-69-1)	99	45	41	<45	58	68	<35	<37
PCB-1260 (11096-82-5)	<28	<24	<35	<45	46	<39	<35	<37

Table 7
Sediment sample particle size characteristics, concentrations (mg/kg dry weight) of heavy metals and percent total organic carbon for the sediments of the Black River Study Area,
1996 and 1997a.

DRAFT
River Mile	Particle Size Characteristics	Percent TOC	Percent Solids	Arsenic	Barium	Cadmium	Chromium

Black River
0.19b	Sand/Silt	2.0	45.6	NA	79.7	3.4	26.3
0.42	Sand/Silt	1.9	54.8	8.73	231	1.3	23.6
2.32	Silt/Sand	1.9	52.6	16.5	174	10.4c	63.2
2.90	Silt/Sand	1.9	44.8	9.25	136	3.69	45.4
4.80	Silt/Sand	2.2	39.8	15.6	274	4.94	74.1
5.20	Sand/Silt	1.4	52.8	9.77	153	3.41	45.4
5.80b	Sand/Silt	1.2	56.5	NA	40.2	3.1	22.5
9.81	Sand	1.3	73.8	9.79	71.9	5.06	60.3
11.60	Sand/Silt	1.1	68.4	10.5	102	60.5	55.9
W. Branch Black River
19.6b	Sand/Silt	0.5	69.4	NA	22.8	0.1	5.4
E. Branch Black River
11.40b	Sand/Silt	1.0	43.1	NA	52.8	0.2	10.1
18.90	Sand	0.5	85.6	6.13	62.6	<0.0882	15
30.00b	Sand/Silt	1.0	58.8	NA	58.3	0.2	9.8
32.50	Sand	0.3	84.0	4.03	28.8	0.114	<13.7
Willow Creek
2.90	Sand	1.1	76.8	9.29	98	0.23	15.3
French Creek
3.20b	Sand	0.1	68.7	NA	15.9	0.4	4.9
6.10b	Sand	0.2	69.7	NA	22.0	0.2	4.7
Key to data: Non-elevated; Slightly elevated; Elevated; Highly elevated; Extremely elevatedd
a Samples collected in 1997 unless otherwise noted. b Samples collected in 1996 c Exceeds Ontario severe effect level (Persuad, et al., 1993) d Data comparisons are made to Ohio EPA reference sites except for mercury for which Illinois stream data are used for comparison (Illinois EPA, 1984)

DRAFT
River Mile	Copper	Iron	Lead	Mercury	Nickel	Zinc
Black River
0.19b	248.4c	120,830	35.9	0.129	29.8	757.3
0.42	20.8	24,900	100	0.0506	<28.6	137
2.32	39.9	57,100	67.4	0.166	35.6	402
2.90	40.6	26,900	50.3	0.224	34.9	171
4.80	52.87	37,000	56.2	0.0925	51.1	245
5.20	35.6	24,700	40.2	0.0756	35.6	158
5.80b	131.6	65,957	23.4	0.0852	21.9	430.5
9.81	48.7	17,100	39.3	0.116	33.7	126
11.60	48.7	29,300	30.3	0.196	36.9	134
W. Branch Black River
19.60b	7.1	11,324	5.9	<0.0301	9.4	36.3
E. Branch Black River
11.40b	36.8	46,666	10.9	0.0320	15.9	149.9
18.90	11.5	18,400	<17.6	0.0507	<17.6	43.2
30.00b	8.0	16,480	10.1	0.0357	16.5	50.1
32.50	<4.58	8,390	<18.3	<0.0192	<18.3	42.6
Willow Creek
2.90	11.7	16,300	26	<0.0282	<20.4	63.8
French Creek
3.20b	41.8	47,624	7.8	0.0232	10.9	221.0
6.10b	38.7	15,802<